Rapid identification of 22 abused drugs and organophosphorus pesticides in blood by LC-MS/MS / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To develop a method for rapid identification of 22 abused drugs and organophosphorus pesticides in the blood.</p><p><b>METHODS</b>Liquid chromatography-tandem mass spectrometry (LC-MS/MS) in multiple-reaction monitoring mode (MRM) was employed for detecting the drugs and pesticides in the blood. The MRM database and criteria for identification were established, and ethyl acetate was used for extraction of the drugs. After 3 rounds of extractions of the blood sample (1 mL) using 2 mL ethyl acetate, the extract was vortexed for 3 min and centrifuged at 5000 r/min. Each organic phase was combined and evaporated by gentle N2 gas. The residue was re-dissolved in 100 L mobile phase, from which 5 L was taken for LC-MS/MS detection.</p><p><b>RESULTS</b>The detection of the 22 target compounds could be completed within 10 min. The limit of detection of the target compound ranged from 0.03 to 6.00 ng/ml. Satisfactory results were obtained in proficiency testing program organized by China National Accreditation Service for Conformity Assessment.</p><p><b>CONCLUSION</b>The method we established is rapid, selective and sensitive for detecting the 22 abused drugs and organophosphorus pesticides.</p>