Primary study on suspension cell culture and polysaccharides content of Achyranthes bidentata / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the relationship between the condition of cell suspension culture and the polysaccharides content in Achyranthes bidentata.</p><p><b>METHOD</b>The methods of orthogonal test and alone factor test were used to study the effect of culture time, inoculation concentration, carbon source and pH.</p><p><b>RESULT AND CONCLUSION</b>The results showed that the optimum medium to induce polysaccharides in A. bidentata was 1/4MS + 6-BA 0.5 + 2,4-D 1.0 mg x L(-1) + sucrose 2% + glucose 2% + fructose 1% cultured after 50 d. The suitable pH was 6.0, and the optimum inoculation concentration was 7g x L(-1).</p>