Chimeric molecules facilitate the degradation of androgen receptors and repress the growth of LNCaP cells / 亚洲男科学杂志(英文版)
Asian Journal of Andrology
; (6): 119-126, 2009.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-284723
Responsible library:
WPRO
ABSTRACT
Post-translational degradation of protein plays an important role in cell life. We employed chimeric molecules (dihydrotestosterone-based proteolysis-targeting chimeric molecule [DHT-PROTAC]) to facilitate androgen receptor (AR) degradation via the ubiquitin-proteasome pathway (UPP) and to investigate the role of AR in cell proliferation and viability in androgen-sensitive prostate cancer cells. Western blot analysis and immunohistochemistry were applied to analyse AR levels in LNCaP cells after DHT-PROTAC treatment. Cell counting and the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) cell viability assay were used to evaluate cell proliferation and viability after AR elimination in both LNCaP and PC-3 cells. AR was tagged for elimination via the UPP by DHT-PROTAC, and this could be blocked by proteasome inhibitors. Degradation of AR depended on DHT-PROTAC concentration, and either DHT or an ALAPYIP-(arg)(8) peptide could compete with DHT-PROTAC. Inhibition of cell proliferation and decreased viability were observed in LNCaP cells, but not in PC-3 or 786-O cells after DHT-PROTAC treatment. These data indicate that AR elimination is facilitated via the UPP by DHT-PROTAC, and that the growth of LNCaP cells is repressed after AR degradation.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Prostatic Neoplasms
/
Dihydrotestosterone
/
Recombinant Fusion Proteins
/
Signal Transduction
/
Receptors, Androgen
/
Cell Survival
/
Ubiquitin
/
Cell Line, Tumor
Limits:
Humans
/
Male
Language:
English
Journal:
Asian Journal of Andrology
Year:
2009
Document type:
Article