The clinical application of spectral karyotyping in the analysis of chromosomal abnormalities / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
; (6): 80-83, 2007.
Article
in Zh
| WPRIM
| ID: wpr-285028
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate value of spectral karyotyping (SKY) in the detection of chromosomal abnormalities.</p><p><b>METHODS</b>A total of 17 metaphase chromosome samples were investigated by SKY, including 10 normal and 5 balanced translocation samples of peripheral blood lymphocytes, one der(Y) sample of peripheral blood lymphocytes and one marker chromosome sample of amniotic fluid cells. The results were compared with those of G-banding diagnosis.</p><p><b>RESULTS</b>Ten normal and 5 balanced translocation samples were diagnosed successfully by SKY in accordance with the results of G-banding; furthermore, SKY analysis revealed that the der(Y) fragment originated from p-arm of chromosome 21 while the marker chromosome originated from chromosome 5.</p><p><b>CONCLUSION</b>SKY is a very sensitive and specific whole genome analysis tool for chromosomal abnormality diagnosis, and exceedingly valuable in the diagnosis on complex chromosomal abnormalities that can not be determined by G-banding.</p>
Full text:
1
Database:
WPRIM
Main subject:
Prenatal Diagnosis
/
Reproducibility of Results
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Chromosome Aberrations
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Chromosome Banding
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Sensitivity and Specificity
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Chromosome Disorders
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Spectral Karyotyping
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Diagnosis
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Genetics
/
Methods
Type of study:
Diagnostic_studies
Limits:
Female
/
Humans
/
Pregnancy
Language:
Zh
Journal:
Chinese Journal of Medical Genetics
Year:
2007
Document type:
Article