A novel method for multiplex detection of gastroenteritis-associated viruses / 病毒学报
Chinese Journal of Virology
; (6): 288-293, 2011.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-286041
Responsible library:
WPRO
ABSTRACT
To develop and optimize a simultaneous detection method of RotavirusA, Norovirus GI, GII, Sapovirus, human astrovirus, enteric adenoviruses and HBoV2 with GenomeLab GeXP analysis system. The sensitivity was verified to be 10(4) copies/microL with plasmids containing the viral targets in triplicate on different days, and no cross-reaction with enterovirus71, human Parechovirus and PicobirnavirusII was observed. Finally, we successfully developed a high throughout, rapid and maneuverable multiplex RT-PCR assay for simultaneous detection of seven viruses related with viral gastroenteritis, which provide a novel method for the molecular diagnosis of diarrhea-associated virus.
Full text:
Available
Health context:
Neglected Diseases
Health problem:
Diarrhea
Database:
WPRIM (Western Pacific)
Main subject:
Virology
/
Viruses
/
Sensitivity and Specificity
/
Reverse Transcriptase Polymerase Chain Reaction
/
Gastroenteritis
/
Methods
Type of study:
Diagnostic study
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Virology
Year:
2011
Document type:
Article