Genotype distribution of enterovirus group C in Shandong Province, China / 病毒学报
Chinese Journal of Virology
; (6): 363-367, 2010.
Article
in Zh
| WPRIM
| ID: wpr-286111
Responsible library:
WPRO
ABSTRACT
Human Enterovirus C group (HEV-C) includes 17 serotypes, which can not be serotype-identified by neutralization test using antiserum pool for NPEV. In order to elucidate the genotypes and molecular evolution of HEV-C in Shandong Province, We selected the strains isolated from AFP cases between 1994-2009 to perform reverse transcriptase-polymerase chain reactions (RT-PCR) by the primers specific for entire VP1 coding gene of HEV-C and sequencing. The phylogenetic tree was then constructed among these VP1 nucleotide sequences and other prototype strains. Totally 12 Shandong local strains were obtained and separated into 4 genotypes, CVA20, CVA21,CVA24 and EV 96. The homologous comparison and phylogenetic analysis showed Shandong strains were distinct from prototype strains in each genotype. This report showed that different genotype HEV-C strains spread widely in Shandong Province.
Full text:
1
Database:
WPRIM
Main subject:
Phylogeny
/
Molecular Sequence Data
/
Cell Line
/
China
/
Classification
/
Reverse Transcriptase Polymerase Chain Reaction
/
Enterovirus C, Human
/
Genetics
/
Genotype
Limits:
Humans
Country/Region as subject:
Asia
Language:
Zh
Journal:
Chinese Journal of Virology
Year:
2010
Document type:
Article