The application of co-culture system on the in vitro development of bovine somatic nuclear transferred embryos / 生物工程学报
Chinese Journal of Biotechnology
; (12): 306-310, 2006.
Article
in Zh
| WPRIM
| ID: wpr-286291
Responsible library:
WPRO
ABSTRACT
To establish a co-culture system of nuclear transferred embryos in bovine, effects of co-culture cell types, passages and cryopreservation as well as addition of BFF or FBS were investigated. The results showed that embryos co-cultured with oviductal epithelial cell and granulosa cell achieved significantly higher blastocyst rate compared with the control group (P < 0.05) and co-cultured with oviductal epithelial cell had more embryo cell number than those with granulosa cell. Passages of co-culture cells significantly affected the blastocyst rate and embryo cell number (P < 0.05), and cryopreservation decreased the blastocyst rate and embryo cell number remarkably. Supplemention of BFF increased blastocyste rate significantly (P < 0.05). In conclusion, co-cultured with fresh primary oviductal epithelial cell along with addition of 10% BFF in SOFaa could improve development of nuclear transferred bovine embryo in vitro.
Full text:
1
Database:
WPRIM
Main subject:
Coculture Techniques
/
Cloning, Organism
/
Culture Media
/
Cell Biology
/
Embryonic Development
/
Embryo, Mammalian
/
Epithelial Cells
/
Nuclear Transfer Techniques
/
Cellular Reprogramming
/
Fallopian Tubes
Limits:
Animals
Language:
Zh
Journal:
Chinese Journal of Biotechnology
Year:
2006
Document type:
Article