Expression of human LNalpha4LG4-5 in Pichia yeast / 生物工程学报
Chinese Journal of Biotechnology
; (12): 226-230, 2006.
Article
in Zh
| WPRIM
| ID: wpr-286304
Responsible library:
WPRO
ABSTRACT
To carry out the secretive expression of human laminin alpha4 chain LG4-5 module (hLNalpha4LG4-5), recombinant expression plasmid pPICZalphaA-LG45 was constructed by inserting of hLNalpha4LG4-5 cDNA into yeast expression vector pPICZaA. The hLNalpha4LG4-5 protein was expressed in GS115 Pichia yeast strain after induced by methanol, and purified target protein can obviously promote the expansion and adhesion of 293 cells.
Full text:
1
Database:
WPRIM
Main subject:
Pichia
/
Plasmids
/
Recombinant Fusion Proteins
/
Cell Line
/
Laminin
/
Genetic Vectors
/
Genetics
/
Metabolism
Limits:
Humans
Language:
Zh
Journal:
Chinese Journal of Biotechnology
Year:
2006
Document type:
Article