Effects of endogenous carbon monoxide on gene expression profiles associated with the apoptosis of pulmonary arterial smooth muscle cells / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
; (12): 882-885, 2010.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-286958
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To identify the gene expression profiles associated with the apoptosis of pulmonary arterial smooth muscle cells stimulated by carbon monoxide (CO).</p><p><b>METHODS</b>Primary cultured Sprague-Dawley rat pulmonary arterial smooth muscle cells (PASMC) were stimulated by platelet-derived growth factor (PDGF, 20 ng/mL) and hemin (20 μmol/L). Cells were harvested after 2 hrs and Affymetrix microarrays were used to detect the gene expression profile.</p><p><b>RESULTS</b>Some genes associated with Map2k3 (P38) signal pathway, such as CyclinD1, CyclinH, CyclinL1, MAP2K3, Kras and Nras, were upregulated, but P27 expression was downregulated after PDGF treatment. After endogenous CO treatment, some genes associated with P53 pathway, such as Gadd45α, P21 and Trp53inp1, were upregulated.</p><p><b>CONCLUSIONS</b>P53 pathway probably plays an important role in apoptosis of pulmonary arterial smooth muscle cells treated with endogenous CO.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
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Physiology
/
Pulmonary Artery
/
Carbon Monoxide
/
Signal Transduction
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Tumor Suppressor Protein p53
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Rats, Sprague-Dawley
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Apoptosis
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Gene Expression Profiling
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Contemporary Pediatrics
Year:
2010
Document type:
Article