Effect of injection salvia miltiorrhiza on the expression of inducible nitric oxide synthase in the cochlea of guinea pig damaged by streptomycin / 中国应用生理学杂志

ABSTRACT

<p><b>AIM</b>To investigate the expression of Inducible Nitric Oxide Synthase (iNOS) in the cochlea of guinea pig after streptomycin (SM) and the antagonism of Salvia Miltiorrhiza injection on SM ototoxicity.</p><p><b>METHODS</b>Light microscope, transmission electron microscope (TEM), immunohistochemical staining and image quantitative analysis technique, combined with auditory brainstem response (ABR) measurement were used.</p><p><b>RESULTS</b>After 10 days by drugs, the threshold of ABR of SM increased significantly, and the threshold of ABR of DS+ SM reduced more than that of SM (P < 0.01), the Corti's organ (CO), inner hair cell (IHC) and outer hair cell (OHC), spiral ganglion(SG), stria vascularis (SV) of SM damaged more greatly than that of DS + SM under light microscope and TEM. The result of immunohistochemical staining implied the expression of iNOS in CO, IHC and OHC, SG, SV of SM higher than that of DS+ SM.</p><p><b>CONCLUSION</b>The threshold of ABR increases and the expression of iNOS strengthens on SM ototoxicity. DS can reduce the increasing and inhibit the over-expression of iNOS, and reduce the damage of SM ototoxicity, which implies the protective role of DS on SM ototoxicity.</p>