Your browser doesn't support javascript.
loading
Jinmaitong alleviates the diabetic peripheral neuropathy by inducing autophagy / 中国结合医学杂志
Article in English | WPRIM (Western Pacific) | ID: wpr-287182
Responsible library: WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To observe the deregulation of autophagy in diabetic peripheral neuropathy (DPN) and investigate whether Jinmaitong ( JMT) alleviates DPN by inducing autophagy.</p><p><b>METHODS</b>DPN models were established by streptozotocin-induced diabetic rats and Schwann cells (SCs) cultured in high glucose medium. The pathological morphology was observed by the improved Bielschowsky's nerve fiber axonal staining and the Luxol fast blue-neutral red myelin staining. The ultrastructure was observed by the transmission electron microscopy. Beclin1 level was detected by immunohistochemistry and Western blot. The proliferation of cultured SCs was detected by methylthiazolyldiphenyl-tetrazolium bromide.</p><p><b>RESULTS</b>Diabetic peripheral nerve tissues demonstrated pathological morphology and reduced autophagic structure, accompanied with down-regulation of Beclin1. JMT apparently alleviated the pathological morphology change and increased the autophagy [in vivo, Beclin1 integral optical density (IOD) value of the control group 86.6±17.7, DM 43.9±8.8, JMT 73.3 ±17.8, P<0.01 or P<0.05, in vitro Beclin1 IOD value of the glucose group 0.47±0.25 vs the control group 0.88±0.29, P<0.05]. Consequently, inhibition of autophagy by 3-methyladenine resulted in a time- and concentration-dependent decrease of the proliferation of SCs (P<0.05, P<0.01).</p><p><b>CONCLUSIONS</b>Down-regulation of autophagy in SCs might contribute to the pathogenesis of DPN. JMT alleviates diabetic peripheral nerve injury at least in part by inducing autophagy.</p>
Subject(s)

Full text: Available Database: WPRIM (Western Pacific) Main subject: Pathology / Pharmacology / Schwann Cells / Sciatic Nerve / Autophagy / Staining and Labeling / Axons / Drugs, Chinese Herbal / Immunohistochemistry / Down-Regulation Limits: Animals Language: English Journal: Chinese journal of integrative medicine Year: 2016 Document type: Article
Full text: Available Database: WPRIM (Western Pacific) Main subject: Pathology / Pharmacology / Schwann Cells / Sciatic Nerve / Autophagy / Staining and Labeling / Axons / Drugs, Chinese Herbal / Immunohistochemistry / Down-Regulation Limits: Animals Language: English Journal: Chinese journal of integrative medicine Year: 2016 Document type: Article
...