Glypican 3 expression in hepatoblastoma and its diagnostic implication / 中华病理学杂志
Chinese Journal of Pathology
; (12): 806-809, 2013.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-288202
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the expression and diagnostic significance of glypican-3 (GPC3) in hepatoblastoma.</p><p><b>METHODS</b>Five tissue microarray paraffin blocks were constructed to include 54 cases of hepatoblastoma. The tumor tissue samples were obtained from 3 surgical biopsies, 33 needle biopsies, 5 stage I resection tumors, and 13 stage II resection tumors after transcatheter arterial chemoembolization. Ten samples of non-neoplastic hepatic tissue adjacent to tumor were used as control. Immunohistochemical staining of GPC3 (clone 1G12) was performed. Among the 54 cases of hepatoblastoma, 22 cases were fetal subtype, 24 cases were mixed fetal and embryonal subtype and 8 cases were mixed epithelial and mesenchymal type.</p><p><b>RESULTS</b>GPC3 was positive in fetal epithelial cells (54/54, 100%), but negative or weakly positive in embryonic epithelial cells in all cases of hepatoblastoma. Undifferentiated small cells and all mesenchymal components were negative for the expression. Non-neoplastic hepatocytes adjacent to tumor were negative for GPC3 expression (0/10) .</p><p><b>CONCLUSIONS</b>Fetal epithelial components of hepatoblastoma express GPC3 protein detectable by immunohistochemistry. Normal hepatocytes after birth, small cell undifferentiated and embryonic epithelial components of hepatoblastoma do not or weakly express GPC3 protein. Therefore, GPC3 immunohistochemistry offers a valuable aid to the diagnosis of hepatoblastoma in infants and children.</p>
Full text:
Available
Health context:
SDG3 - Health and Well-Being
/
SDG3 - Target 3.4 Reduce premature mortality due to noncommunicable diseases
Health problem:
Target 3.2: Reduce avoidable death in newborns and children under 5
/
Digestive System Diseases
/
Liver Cancer
Database:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Immunohistochemistry
/
Hepatoblastoma
/
Diagnosis
/
Diagnosis, Differential
/
Epithelial Cells
/
Glypicans
/
Liver Neoplasms
/
Metabolism
Type of study:
Diagnostic study
Limits:
Child
/
Child, preschool
/
Female
/
Humans
/
Infant
/
Male
/
Infant, Newborn
Language:
Chinese
Journal:
Chinese Journal of Pathology
Year:
2013
Document type:
Article