Changes in levels of TNF-α and GFAP in brain and spinal cord of rabbits with decompression disease / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
; (12): 607-609, 2014.
Article
in Zh
| WPRIM
| ID: wpr-289845
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the changes in expression of tumor necrosis factor-alpha (TNF-α) and glial fibrillary acidic protein (GFAP) in rabbits with decompression disease (DCS), and to investigate the functioning mechanism.</p><p><b>METHODS</b>A total of 21 healthy adult rabbits were randomly divided into 3 groups: normal control group, DCS group, and safe relief group, with 7 rabbits in each group. A rabbit DCS model was established by quick decompression. The changes in pathological morphology and mRNA and protein expression of TNF-α and GFAP in the brain and spinal cord of rabbits with DCS were determined by light microscopy, real-time PCR, and immunohistochemistry, respectively.</p><p><b>RESULTS</b>Cavity formation was observed in the white matter of spinal cord in DCS group. The mRNA and protein expression of TNF-α and GFAP was significantly higher in the DCS group than in the normal control group and safe relief group (P < 0.01), while no significant differences were observed in the brain (P > 0.05).</p><p><b>CONCLUSION</b>Spinal cord is the main part of central nervous system injury in DCS. Activation of TNF-α and GFAP genes accompanied by increase in their protein expression can be observed at the early stage of DCS. The astrocytes and TNF-α play important roles in the process of spinal cord injury in DCS.</p>
Full text:
1
Database:
WPRIM
Main subject:
Spinal Cord
/
Brain
/
Tumor Necrosis Factor-alpha
/
Decompression Sickness
/
Disease Models, Animal
/
Glial Fibrillary Acidic Protein
/
Metabolism
Type of study:
Prognostic_studies
Limits:
Animals
Language:
Zh
Journal:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Year:
2014
Document type:
Article