Culturing Schwann Cells from Neonatal Rats by Improved Enzyme Digestion Combined with Explants-culture Method / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
; (6): 388-392, 2016.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-289848
Responsible library:
WPRO
ABSTRACT
Objective To develop an improved method for culturing Schwann cells(SCs) by using both enzyme digestion and explants-culture approaches and compared with traditional explants-culture method and general hemi-explants-culture method. Methods Bilaterally sciatic nerves and brachial plexus nerves were dissected from 3 to 5-day-old neonatal SD rats and explants-culture method,general hemi-explants-culture method,and improved enzyme digestion combined with explants-culture method were adopted to culture SCs,respectively. SCs were digested and passaged after 7 days in culture and counted under the microscope. The purity of SCs was identified by S-100 immunofluorescence staining. Results The SCs of improved method group grew fastest and the total number of cells obtained was(1.85±0.13)×10(6);the SCs of the hemi-explants-culture method group grew slower than the improved method group and the total number of cells obtained was (1.10±0.10)×10(6);the SCs of the explants-culture method group grew slowest and the total number of cells obtained was (0.77±0.03)×10(6).The total number of cells obtained showed significant difference among the three groups(P<0.01). Immunofluorescence staining showed that the SCs purity was (95.73±1.51)% in the improved method group,(84.66±2.68)% in the hemi-explants-culture method group,and (74.50±4.23)% in the explants-culture method group(P<0.01). Conclusion The improved enzyme digestion combined with explants-culture method can obtain sufficient amount of high-purity SCs in a short time and thus may be applied in further research on peripheral nerve regeneration.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Schwann Cells
/
Sciatic Nerve
/
Brachial Plexus
/
Cell Separation
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Cells, Cultured
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Rats, Sprague-Dawley
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Cell Culture Techniques
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Cell Biology
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Enzymes
/
Animals, Newborn
Limits:
Animals
Language:
English
Journal:
Acta Academiae Medicinae Sinicae
Year:
2016
Document type:
Article