Arginine vasopressin-induced nitric oxide content changes in cultured cardiac fibroblasts and its relation to nuclear factor-kappaB / 生理学报
Acta Physiologica Sinica
; (6): 417-421, 2003.
Article
in Zh
| WPRIM
| ID: wpr-290950
Responsible library:
WPRO
ABSTRACT
To investigate the changes in the nitric oxide (NO) contents, nitric oxide synthase (NOS) activity and inducible nitric oxide (iNOS) mRNA expression in arginine vasopressin (AVP)-induced cardiac fibroblasts (CFs) in vitro and its relation to nuclear factor-kappaB (NF-kappaB), CFs were isolated by trypsin digestion method. Nitric acid reductase method, spectrophotometry, reverse transcription-polymerase chain reaction (RT-PCR), immunofluorescence-interactive laser cytometer techniques and Western blotting were used respectively to detect NO contents, NOS activity, iNOS mRNA expression and the activation of NF-kappaB in CFs. AVP increased NO contents, NOS activity and iNOS mRNA expressions in a concentration-dependent manner; NF-kappaB was activated and mobilized from cytoplasm to nucleus in AVP-induced CFs; PDTC, one of the inhibitors of NF-kappaB, could inhibit aforementioned increments. It is suggested that the increases in NO contents, elevation of NOS activity and increment of iNOS mRNA expression may be mediated through NF-kappaB activation pathway in cultured CFs induced by AVP, and that NF-kappaB is involved in the occurrence and development of myocardial fibrosis.
Full text:
1
Database:
WPRIM
Main subject:
Pharmacology
/
RNA, Messenger
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Arginine Vasopressin
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Cells, Cultured
/
NF-kappa B
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Rats, Sprague-Dawley
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Cell Biology
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Myocytes, Cardiac
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Nitric Oxide Synthase Type II
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Fibroblasts
Limits:
Animals
Language:
Zh
Journal:
Acta Physiologica Sinica
Year:
2003
Document type:
Article