Protective effect of isoorientin on alcohol-induced hepatic fibrosis in rats / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 3726-3730, 2013.
Article
in Zh
| WPRIM
| ID: wpr-291295
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effect and mechanism of isoorientin from Gypsophila elegans on alcohol-induced hepatic fibrosis in rats.</p><p><b>METHOD</b>ninety healthy male Wistar rats were randomly divided into six groups: the normal control group, the model control group, the colchicines group (positive control, 1.0 mg x kg(-1) x d(-1)), the high, middle and low-dose isoorientin groups (20, 50, 100 mg x kg(-1) x d(-1)). The normal control group received normal saline, while other groups received alcohol to cause hepatic fibrosis. After 24-weeks treatment, the alanine aminotransferase (ALT), aspartate aminotransferase (AST), Interleukin 6 (IL-6), tumor necrosis factor-alpha (TNF-alpha), hyaluronic acid (HA), laminin (LN), type III precollagen (PCIII), hydroxyproline (Hyp), Myeloperoxidase (MPO), malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) were assayed according to the manufacturer's instructions, the alpha-SMA and TGF-beta1 were detected by western blotting, and the histopathological changes was observed by H&E staining.</p><p><b>RESULT</b>Isoorientin could improve the liver function by decreasing the activity of ALT, AST, IL-6, TNF-alpha, MDA, MPO, HA, LN, PCIII and Hyp (P < 0.05), increasing the activity of SOD and GSH-Px (P < 0.05), and reducing the expression of alpha-SMA and TGF-beta1 (P < 0.05). In addition, the high and middle-dose isoorientin groups showed more remarkable effect</p><p><b>CONCLUSION</b>Isoorientin from G. elegans can protect hepatic fibrosis induced by alcohol.</p>
Full text:
1
Database:
WPRIM
Main subject:
Aspartate Aminotransferases
/
Blood
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Drugs, Chinese Herbal
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Rats, Wistar
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Protective Agents
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Luteolin
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Alanine Transaminase
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Ethanol
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Drug Therapy
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Transforming Growth Factor beta1
Type of study:
Prognostic_studies
Limits:
Animals
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Humans
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Male
Language:
Zh
Journal:
China Journal of Chinese Materia Medica
Year:
2013
Document type:
Article