Study on the T lymphocytes early activation and soluble tumor necrosis factor receptor in patients with aplastic anemia / 中华血液学杂志
Chinese Journal of Hematology
; (12): 209-212, 2004.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-291439
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression of T cell early activation marker (CD(69)) on peripheral CD(4)(+) and CD(8)(+) lymphocytes and serum levels of soluble tumor necrosis factor receptor 1 and 2 (sTNF-R1 and sTNF-R2) in serum and bone marrow in patients with aplastic anemia (AA) and their pathophysiological significance.</p><p><b>METHODS</b>In vitro activation of T lymphocytes was carried out by whole blood cell culture containing PHA (20 micro g/ml). The CD(69) expressions on CD(4)(+) and CD(8)(+) lymphocytes at 0 h and 4 h after PHA exposure were analyzed by two-color flow cytometry. The levels of sTNF-R1 and sTNF-R2 in serum and bone marrow were measured by ELISA.</p><p><b>RESULTS</b>The CD(69) expression rates of CD(4)(+) and of CD(8)(+) cells in SAA patients were (8.96 +/- 7.23)% and (10.67 +/- 7.58)%, respectively, and that of CD(8)(+) cells in CAA patients was (7.36 +/- 5.49)% before PHA stimulation. The CD(69) expression rates of CD(4)(+) and of CD(8)(+) cells in SAA patients were (71.73 +/- 11.91)% and (61.74 +/- 13.44)% and in CAA (59.35 +/- 10.15)% and (48.78 +/- 8.25)% respectively, and were significantly elevated after PHA stimulation. CD(69) expression on CD(4)(+) cells was much higher than that on CD(8)(+) cells after stimulation. The levels of the two sTNF-R (sTNF-R1 and sTNF-R2) in peripheral blood and bone marrow of SAA patients were elevated and in the bone marrow of CAA patients were also increased. The serum levels of sTNF-R2 were positively related to the CD(69) expression rates of CD(8)(+) cells before PHA stimulation.</p><p><b>CONCLUSION</b>Increased early activation and activated potentials of T lymphocytes, along with abnormally elevated immunologically active molecules might play a major role in the pathogenesis of AA.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Blood
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CD4-Positive T-Lymphocytes
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Antigens, Differentiation, T-Lymphocyte
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Antigens, CD
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Chemistry
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Receptors, Tumor Necrosis Factor
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CD8-Positive T-Lymphocytes
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Lectins, C-Type
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Receptors, Tumor Necrosis Factor, Type I
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Receptors, Tumor Necrosis Factor, Type II
Limits:
Adolescent
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Adult
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Aged
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Child
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Female
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Humans
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Male
Language:
Chinese
Journal:
Chinese Journal of Hematology
Year:
2004
Document type:
Article