Minimal residual disease monitoring in chronic myeloid leukemia patients after allogeneic hematopoietic stem cell transplantation using interphase fluorescence in situ hybridization and real-time quantitative reverse transcription PCR / 癌症
Chinese Journal of Cancer
; (12): 194-197, 2010.
Article
in En
| WPRIM
| ID: wpr-292611
Responsible library:
WPRO
ABSTRACT
<p><b>BACKGROUND AND OBJECTIVE</b>Interphase fluorescence in situ hybridization (FISH) and real-time quantitative reverse transcription PCR (RQ-PCR) are the common methods for monitoring minimal residual disease (MRD) in chronic myeloid leukemia (CML) patients. This study was to assess the value of monitoring BCR-ABL fusion gene level in CML patients after allogeneic hematopoietic stem cell transplantation (allo-HSCT) using FISH and RQ-PCR.</p><p><b>METHODS</b>BCR-ABL fusion gene levels were detected in the bone marrow of 31 patients with CML before and 3-48 months after allo-HSCT using FISH and RQ-PCR.</p><p><b>RESULTS</b>BCR-ABL positive cells detected by FISH were decreased 3-30 months after allo-HSCT and BCR-ABL/ABL mRNA was reduced by 2 logarithmic units in RQ-PCR (P < 0.05). While no BCR-ABL positive cell was detected by FISH 30 months after allo-HSCT, BCR-ABL/ABL mRNA was detected by RQ-PCR and declined by more than 3 logarithmic units, (P < 0.05).</p><p><b>CONCLUSIONS</b>Dynamic monitoring of BCR-ABL gene on molecular level in CML patients after allo-HSCT is useful in the early prediction of susceptibility to recurrence in the patients and in designing intervention, and is thus helpful in improving the overall survival rate after transplantation.</p>
Full text:
1
Database:
WPRIM
Main subject:
Pathology
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Therapeutics
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RNA, Messenger
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Leukemia, Myelogenous, Chronic, BCR-ABL Positive
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Fusion Proteins, bcr-abl
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In Situ Hybridization, Fluorescence
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Neoplasm, Residual
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Hematopoietic Stem Cell Transplantation
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Reverse Transcriptase Polymerase Chain Reaction
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Genetics
Limits:
Adolescent
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Adult
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Child
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Female
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Humans
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Male
Language:
En
Journal:
Chinese Journal of Cancer
Year:
2010
Document type:
Article