Quantitative Measurement of Serum MicroRNA-21 Expression in Relation to Breast Cancer Metastasis in Chinese Females
Annals of Laboratory Medicine
; : 226-232, 2015.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-29326
Responsible library:
WPRO
ABSTRACT
BACKGROUND:
Breast cancer is the most common type of cancer in females. Aberrant expression of microRNA-21 (miR-21) has previously been reported in breast cancer tissue. The aim of this study was to investigate expression levels of serum miR-21 in breast cancer patients and evaluate its prognostic value in Chinese females.METHODS:
Real-time quantitative (RQ)-PCR was used to analyze miR-21 expression in archived serum, tumor tissue, and adjacent normal tissue from 549 participants (326 with breast cancer, 223 without breast cancer). We also analyzed associations between serum miR-21 expression and breast cancer subtypes and patient prognosis. Recurrence and survival were analyzed by using the multivariate Cox proportional hazards model.RESULTS:
Expression of miR-21 was significantly higher in breast cancer tissues compared with normal adjacent breast tissues (P<0.001). The 2(-DeltaDeltaCt) values for serum miR-21 in breast cancer patients versus healthy controls were 9.12+/-3.43 and 2.96+/-0.73, respectively. Multivariate Cox proportional hazards model suggested that serum miR-21 expression was an independent poor prognostic factor for both recurrence (hazard ratio [HR]= 2.942; 95% confidence interval [CI]=1.420-8.325; P=0.008) and disease-free survival (HR=2.732; 95% CI=1.038-7.273, P=0.003) in breast cancer.CONCLUSIONS:
Increased serum miR-21 expression level was correlated with poor prognosis of breast cancer patients, indicating that serum miR-21 may be a novel prognostic marker for recurrence and survival of breast cancer patients before resection.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Prognosis
/
Breast
/
Breast Neoplasms
/
Biomarkers, Tumor
/
Proportional Hazards Models
/
Disease-Free Survival
/
MicroRNAs
/
Kaplan-Meier Estimate
/
Real-Time Polymerase Chain Reaction
/
Lymphatic Metastasis
Type of study:
Prognostic study
Limits:
Female
/
Humans
Language:
English
Journal:
Annals of Laboratory Medicine
Year:
2015
Document type:
Article