Inhibitory effect of a novel crosslinking quaternary ammonium methacrylates on matrix metalloproteinases / 中华口腔医学杂志
Chinese Journal of Stomatology
; (12): 239-243, 2013.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-293601
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the anti-matrix metalloproteinase (MMP) activity of a novel crosslinking quaternary ammonium methacrylates, 2-methacryloxylethyl dodecylmethyl ammonium bromide (MAE-DB).</p><p><b>METHODS</b>The effects of MAE-DB at different concentrations (1%, 3%, 5%, 7%) on soluble matrix metalloproteinase-8 (MMP-8) were investigated using fluorescent assay kit. Readings were taken every 20 min for 3 h. The 1, 10-phenanthroline provided by the assay kit served as control group. Demineralized dentin beams were randomly divided into three groups (n = 50) and immersed in different solutions artificial saliva, MAE-DB incorporated artificial saliva and chlorhexidine incorporated artificial saliva. After temperature cycling, the changes of ultimate tensile strength were measured to determine the effect of MAE-DB on the activity of matrix-bound endogenous matrix metalloproteinases. The morphology of dentin collagen fibrils in the three groups was observed via transmission electron microscopy (TEM).</p><p><b>RESULTS</b>MAE-DB could effectively inhibit the activity of soluble MMP-8. The inhibition percentage of 3% MAE-DB was 99.53% after 1 h, and it was significantly higher than that of 1, 10-phenanthroline (95.71%, P < 0.05). After temperature cycling, the ultimate tensile strengths of MAE-DB groups were significantly higher than those of the artificial saliva groups and the chlorhexidine groups (P < 0.05). TEM micrographs of MAE-DB group revealed that the microstructure of the collagen fibrillar was intact, while the fibrillar in the artificial saliva group was disrupted, indicating a protective function of MAE-DB on dentin collagen.</p><p><b>CONCLUSIONS</b>MAE-DB can inhibit the activity of MMP and protect dentin collagen from enzyme degradation.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Chemistry
/
Chlorhexidine
/
Matrix Metalloproteinase 8
/
Dentin
/
Ammonium Compounds
/
Methacrylates
Language:
Chinese
Journal:
Chinese Journal of Stomatology
Year:
2013
Document type:
Article