Effect of baicalin on expression of TLR4 in RAW264.7 cells infected by ESBLs Escherichia coli / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 1590-1594, 2013.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-294062
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect and mechanism of baicalin against beta-lactamases Escherichia coli (ESBLs E. coli) mediated by toll-like receptor 4 (TLR4) signal transduction pathway.</p><p><b>METHOD</b>The RAW264. 7 cells monolayer pretreated with different concentration of baicalin were inoculated with ESBLs E. coli. The expression of TLR4 mRNA and protein were analyzed by semi-quantitative RT-PCR and Immunofluorescence, respectively. The activity of NF-kappaB was detected by Western blot using total cellular protein. The production of TNF-alpha in supernatant was determined by enzyme linked immunosorbnent assay (ELISA).</p><p><b>RESULT</b>ESBLs E. coli significantly up-regulated the expression levels of TLR4 mRNA and protein in a time-dependent manner, induced the activation of NF-KB in RAW264. 7, enhanced the production of TNF-alpha in supernatant. Baicalin down-regulated the expression of TLR4 mRNA and protein, decreased the activation of NF-KB in RAW264. 7 cells and reduced the production of TNF-alpha in supernatant in a dose-dependent manner.</p><p><b>CONCLUSION</b>Baicailin could inhibit TLR4 signal transduction pathway. The mechanism of baicalin against ESBLs E. coli may be through inhibiting the expression of TLR4 and its signal transduction pathway.</p>
Full text:
Available
Health context:
SDG3 - Health and Well-Being
/
Neglected Diseases
Health problem:
Target 3.3: End transmission of communicable diseases
/
Neglected Diseases
/
Zoonoses
Database:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Physiology
/
Flavonoids
/
Gene Expression
/
Cell Line
/
Drug Therapy
/
Allergy and Immunology
/
Escherichia coli
/
Escherichia coli Infections
/
Toll-Like Receptor 4
Limits:
Animals
/
Humans
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2013
Document type:
Article