Up-regulation of Fas is related to apoptosis of HeLa cells induced by co-immobilized TNF-alpha/IFN-gamma / 生物医学工程学杂志
Journal of Biomedical Engineering
; (6): 615-619, 2009.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-294606
Responsible library:
WPRO
ABSTRACT
This study was aimed to examine the expression of apoptosis-associated gene Fas in HeLa cell, explore the effects of the co-immobilized cytokines (tumor necrosis factor-alpha and interferon-gamma), and probe the potential mechanism of action. The preparation and application of the research couple IFN-gamma and TNF-alpha to the polystyrene cell culture plate were performed using the Photo-immobilization method, with different doses (20 ng/well and 200 ng/well) and synthesized optical active material. HeLa cells were treated with cytokines for two dose and 1, 3, 6 days. The result showed that the free cytokines induced HeLa apoptosis quickly, yet the HeLa apoptosis induced by co-immobilized cytokines had longer effect.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
HeLa Cells
/
Up-Regulation
/
Chemistry
/
Interferon-gamma
/
Tumor Necrosis Factor-alpha
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Apoptosis
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Fas Receptor
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Drug Synergism
/
Immobilized Proteins
Limits:
Humans
Language:
Chinese
Journal:
Journal of Biomedical Engineering
Year:
2009
Document type:
Article