Adeno-associated virus mediated T-bet gene transfer into SGC-7901 cell to regulate IFN-gamma production / 生物医学工程学杂志
Journal of Biomedical Engineering
; (6): 606-619, 2009.
Article
in Zh
| WPRIM
| ID: wpr-294608
Responsible library:
WPRO
ABSTRACT
In order to investigate the effect of T-bet on malignant cells, we selected SGC-7901, a kind of human gastric carcinoma cell line, and used gene clone technique and adeno-associated virus (AAV) packing technology, thus obtaining a recombinant rAAV-eGFP-T-bet and T-bet gene-transfected SGC-7901 cells. Then the function of T-bet gene-infected SGC-7901 cells was researched by detecting the levels of IFN-gamma and T-bet production. The results showed: (1) It was verified that rAAV-T-bet's packing was completed; (2) After SGC-7901 cells was transfected by rAAV-eGFP-T-bet, a green fluorescence was found in about 30%-40% SGC-7901s, and the gene of 1670 bp (T-bet) and 388 bp (IFN-gamma) were generated from SGC-7901s cells; (3) The proteins of IFN-gamma and T-bet secreted by SGC-7901 cells were also detected. These reveal that SGC-7901 cell is efficiently infected by rAAV encoding T-bet, which can induce transfected cells to secret IFN-gamma. It may be useful in the researches on cancer immune therapy of transfecting T-bet gene.
Full text:
1
Database:
WPRIM
Main subject:
Stomach Neoplasms
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Recombinant Proteins
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Transfection
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Interferon-gamma
/
Dependovirus
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T-Box Domain Proteins
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Cell Line, Tumor
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Green Fluorescent Proteins
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Genetics
/
Metabolism
Limits:
Humans
Language:
Zh
Journal:
Journal of Biomedical Engineering
Year:
2009
Document type:
Article