Sinoatrial node cells phenotype expression is induced by direct bone marrow stem cells contact with sinoatrial node cells / 中华心血管病杂志
Chinese Journal of Cardiology
; (12): 73-76, 2009.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-294775
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To observe the differentiation of bone mesenchymal stem cells (BMSCs) co-cultured with purified sinoatrial node cells (SNC) of neonate rats.</p><p><b>METHODS</b>SNC from neonatal SD rat were cultured and purified with differential attachment method and labeled with BrdU. Rat BMSCs were isolated by a Percoll's gradient solution and cultured in DMEM. After 2 passages, these BMSCs were transfected with pEGFP-N1 by Lipofectamine and labeled with GFP. EGFP-BMSC were co-cultured with SNC in a rate of 15 for 1 week. EGFP-BMSC cultured in SNC culture medium served as controls. SNC marker hyperpolarization activated cyclic nucleotide gated cation channel 4 (HCN4) and connexin 45 (Cx45) expressions were determined by immunofluorescence staining.</p><p><b>RESULT</b>Positive immunofluorescence staining against HCN4 and Cx45 were detected in EGFP-BMSC co-cultured with SNC but not in EGFP-BMSC cultured in SNC culture medium.</p><p><b>CONCLUSION</b>Direct cell-to-cell contact between BMSCs and SNC cells may induce BMSCs differentiation into sinus node-like cells.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Sinoatrial Node
/
Bone Marrow Cells
/
Cell Differentiation
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Cell Separation
/
Cells, Cultured
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Rats, Sprague-Dawley
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Coculture Techniques
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Cell Culture Techniques
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Cell Biology
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Mesenchymal Stem Cells
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Cardiology
Year:
2009
Document type:
Article