Expression of human papillomavirus type 16/18 in human cervical carcinomas by the quantum dot fluorescent in-situ hybridization / 中华病理学杂志
Chinese Journal of Pathology
; (12): 675-677, 2010.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-295155
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate fluorescence in situ hybridization labeled with quantum dots (QDs) for the detection of human papillomavirus 16/18 (HPV16/18) infection in cervical carcinoma patients.</p><p><b>METHODS</b>A total of 80 biopsy samples of squamous carcinoma of cervix were assayed for HPV 16/18 infection by using quantum dot labeled fluorescent in situ hybridization (QD-FISH) and chromogenic in situ hybridization (CISH) techniques, respectively. The results obtained by using two different methods were statistically analyzed.</p><p><b>RESULTS</b>The positive rate for HPV16/18 by QD-FISH was 88.8% (71/80), higher than that (80.0%) by CISH, however, the result was statistically not significant (P=0.127). The positive detection rates for HPV16/18 by using both methods increased coincidentally with raising of the tumor grading stage.</p><p><b>CONCLUSION</b>The sensitivity and specificity of HPV infection detectable by QD-FISH is higher than that by the CISH technique.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Virology
/
Carcinoma, Squamous Cell
/
Uterine Cervical Dysplasia
/
Uterine Cervical Neoplasms
/
Chromogenic Compounds
/
Sensitivity and Specificity
/
In Situ Hybridization, Fluorescence
/
Papillomavirus Infections
/
Quantum Dots
Type of study:
Diagnostic study
Limits:
Adult
/
Female
/
Humans
Language:
Chinese
Journal:
Chinese Journal of Pathology
Year:
2010
Document type:
Article