Detection of BRAF V600E mutation in hairy cell leukemia by high- resolution melting analysis / 中华血液学杂志
Chinese Journal of Hematology
; (12): 207-209, 2014.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-295674
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the feasibility and diagnostic implication of BRAF V600E mutation identified by high-resolution melting (HRM) assay in patients with hairy cell leukemia (HCL).</p><p><b>METHODS</b>The V600E mutation of BRAF exon 15 in four HCL patients were detected by HRM assay and patients' clinical data were retrospectively analyzed.</p><p><b>RESULTS</b>All four HCL patients were positive for the BRAF V600E mutation, which were identical to the results of DNA sequencing.</p><p><b>CONCLUSION</b>The HRM assay for BRAF V600E mutation provides a useful tool to aid the laboratory diagnosis of HCL with easy operability, accuracy, and low cost.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
DNA Mutational Analysis
/
Leukemia, Hairy Cell
/
Polymerase Chain Reaction
/
Proto-Oncogene Proteins B-raf
/
Diagnosis
/
Genetics
/
Methods
/
Mutation
Type of study:
Diagnostic study
Limits:
Adult
/
Aged
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Hematology
Year:
2014
Document type:
Article