Identification of MicroRNA-like Viral Small RNAs from AaeDV / 病毒学报
Chinese Journal of Virology
; (6): 537-541, 2015.
Article
in Zh
| WPRIM
| ID: wpr-296251
Responsible library:
WPRO
ABSTRACT
Several virus families have been shown to encode microRNAs (miRNAs), which have roles in the infection and replication of viruses in host cells. These virus-encoded miRNAs are identified in double-stranded DNA virus (dsDNA virus) and in several RNA virus families, but not in single-stranded DNA virus (ssDNA virus). We used a bioinformatics approach based on VMir, miRNAFold and MaturePred software to predict virus-encoded miRNA-like small RNAs from the genome of a ssDNA virus: Aedes aegypti densovirus (AaeDV). Northern blotting and stem-loop reverse transcription-polymerase chain reaction (RT-PCR) were used to detect predicted small RNAs. A miRNA-like small RNA termed "AaeDVMD" was identified by stem-loop RT-PCR from predicted candidates. This is the first report demonstrating that a ssDNA virus can encode miRNA-like small RNAs. These data will aid further exploration of the interaction between the AaeDV and its mosquito host.
Full text:
1
Database:
WPRIM
Main subject:
Virology
/
RNA, Viral
/
Molecular Sequence Data
/
Base Sequence
/
Chemistry
/
Densovirinae
/
Computational Biology
/
Aedes
/
MicroRNAs
/
Genetics
Limits:
Animals
Language:
Zh
Journal:
Chinese Journal of Virology
Year:
2015
Document type:
Article