Biological transport of minocycline by human periodontal ligament fibroblasts / 华西口腔医学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate biological transport of minocycline by human periodontal ligament fibroblasts (HPDLF). To verify the hypothesis of delivering medicine to periodontium and the whole body through the root canal.</p><p><b>METHODS</b>HPDLF and MC3T3-E1 cells were incubated in minocycline solutions. The intracellular antibiotics contents were measured by high performance liquid chromatography (HPLC) and the cell total protein was measured by Bradford protein assay.</p><p><b>RESULTS</b>HPLC was an accurate, sensitive method for measurement of the intracellular minocycline. The incubation time and cell property had significant effect on the intracellular minocycline contents (P< 0.01). The intracellular contents increased with extracellular concentration.</p><p><b>CONCLUSION</b>Minocycline can be transported by HPDLF. The transport is concentration-dependent, time-dependent and cell specific.</p>