Humoral immune response and in vitro neutralizing antibody assay on co-delivery of protein HPV16L1 virus-like particle with HPV16L1 gene / 中华病理学杂志
Chinese Journal of Pathology
; (12): 421-424, 2005.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-297332
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To compare humoral immune response by co-inoculating mice with antigen HPV16L1 virus-like particle (VLP) and HPV16L1 recombinant plasmids and then observing the neutralizing antibody activity in vitro.</p><p><b>METHODS</b>C57BL/6 mice were injected intramuscularly/subcutaneously with pcDNA-L1 plasmids plus HPV16L1 VLP. Serum IgG levels were detected by ELISA, antibody neutralizing protective activities were determined by hemagglutination inhibition and HPV16L1 VLP binding inhibition assay.</p><p><b>RESULTS</b>Serum antibody titers and neutralizing antibody activities were increased in HPV16L1 plasmids plus HPV16L1 VLP proteins in co-immunized mice when compared with controls.</p><p><b>CONCLUSION</b>Co-inoculation of the HPV16L1 VLP protein can enhance production of neutralizing antibody activities against aimed antigen, which should be a more promising strategy for effective HPV16 prophylactic vaccine development.</p>
Full text:
Available
Health context:
SDG3 - Health and Well-Being
Health problem:
Target 3.3: End transmission of communicable diseases
Database:
WPRIM (Western Pacific)
Main subject:
Plasmids
/
Blood
/
Recombinant Proteins
/
Immunoglobulin G
/
Neutralization Tests
/
HeLa Cells
/
Oncogene Proteins, Viral
/
Immunization
/
Capsid Proteins
/
Allergy and Immunology
Limits:
Animals
/
Humans
Language:
Chinese
Journal:
Chinese Journal of Pathology
Year:
2005
Document type:
Article