Changes of apoptosis, mitochondrion membrane potential and Ca2+ of hypothalamic neurons induced by high power microwave / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
; (12): 739-741, 2006.
Article
in Zh
| WPRIM
| ID: wpr-297580
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the injury effect and mechanism of hypothalamic neurons after high power microwave (HPM) exposure.</p><p><b>METHODS</b>Primarily cultured hypothalamic neurons were exposed to 10 and 30 mW/cm(2) HPM, and the inverted phase contrast microscope (IPCM) and flow cytometry (FCM) were employed to detect the injury of cells and change of mitochondrion membrane potential (MMP) and Ca(2+) in the cytoplasm of neurons.</p><p><b>RESULTS</b>The ratio of apoptosis was significantly higher than that of the sham exposure (P < 0.05) induced by 10 and 30 mW/cm(2) HPM and necrosis increased significantly (P < 0.05) in the group of 30 mW/cm(2) at 6 h after exposure. The content of Ca(2+) in the cytoplasm of neuron cells increased (P < 0.01) while MMP decreased significantly (P < 0.01) after radiation of 30 mW/cm(2) HPM at 6 h after exposure.</p><p><b>CONCLUSION</b>Apoptosis is one of the major death ways of hypothalamic neurons. The overloading of Ca(2+) and the decline of MMP are involved in the process.</p>
Full text:
1
Database:
WPRIM
Main subject:
Radiation Effects
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Cells, Cultured
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Calcium
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Rats, Wistar
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Apoptosis
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Cell Biology
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Membrane Potential, Mitochondrial
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Hypothalamus
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Membrane Potentials
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Metabolism
Limits:
Animals
Language:
Zh
Journal:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Year:
2006
Document type:
Article