Genome sequencing and analysis of a peste des petits ruminants virus isolate, China/Tib/07 / 病毒学报
Chinese Journal of Virology
; (6): 322-329, 2010.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-297863
Responsible library:
WPRO
ABSTRACT
Peste des petits ruminants virus is a member of Morbillivirus Paramyxoviridae. The complete genome of a Peste des petits ruminants virus (PPRV) isolate, China/Tib/07 was sequenced and molecular characteristics was analyzed. The internal sequences of the virus genome were amplified by RT-PCR with primers designed according to the published data in GenBank, while the sequences of the 3' and 5' ends of the genome were determined by RACE. Amplification products were directly sequenced,assembled and analyzed with DNAStar4.0. Results showed that China/Tib/07 genome consisted of 15 948 nucleotides in length, encoding six structural proteins and two non-structural proteins just like other known PPRV genomes. Phylogenetically, the virus genome shared homology of 91.6%-98.1% with Southwest Asian isolates among PPRV strains and the highest homology of 64.3% with rinderpest virus among morbillivirus members.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Phylogeny
/
Sheep Diseases
/
Viral Proteins
/
Virology
/
Vero Cells
/
Sheep
/
Molecular Sequence Data
/
Base Sequence
/
Chlorocebus aethiops
/
China
Limits:
Animals
Country/Region as subject:
Asia
Language:
Chinese
Journal:
Chinese Journal of Virology
Year:
2010
Document type:
Article