Effect of simian vacuolating virus 40 on development and differentiation of dendritic cells from Rhesus macaque / 病毒学报
Chinese Journal of Virology
; (6): 344-348, 2009.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-297950
Responsible library:
WPRO
ABSTRACT
To study the effect of simian vacuolating virus 40 (SV40) on development and differentiation of dendritic cells (DC) from rhesus macaque, the peripheral blood-derived dendritic cells from rhesus monkey were pulsed with inactivated SV40 and infective SV40, respectively at the 5th day post DC cultivation. Expressions of CD1a, HLA-DR, CD86 and CD83 on the cell surface at the 7th, 9th day post DC cultivation were analyzed by flow cytometry (FCM). The results showed that expressions of CD1a, HLA-DR, CD86 and CD83 on the cell surface in the inactivated SV40-pulsed experimental group were higher than those in the infective SV40-pulsed experimental group (P < 0.05). These cell surface molecules represented characteristic development and differentiation phase of DC. Down-regulation of expressions of these cell surface molecules indicated that infective SV40 might hamper differentiation and maturation of dendritic cells from rhesus monkey.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Virology
/
Dendritic Cells
/
Immunoglobulins
/
Membrane Glycoproteins
/
HLA-DR Antigens
/
Antigens, CD
/
Cell Differentiation
/
Cells, Cultured
/
Simian virus 40
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Virology
Year:
2009
Document type:
Article