Experimental study on metabonomics of coronary heart disease myocardial ischemia of blood stasis syndrome based on the NOESY pulse NMR / 中国中西医结合杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study changes of serum metabolite profiles in Ameriod ring induced coronary heart disease (CHD) chronic myocardial ischemia (CMI) mini-pigs by NMR.</p><p><b>METHODS</b>The blood stasis syndrome (BSS) animal model was established by coronary angiography, blood hemorheology, and so on. By using NMR and pattern recognition, changes of serum endogenous metabolites of the four-week mini-pigs of the BSS model and the sham-operative group.</p><p><b>RESULTS</b>The model prepared by using this method was confirmed as the stable CHD CMI model of BSS. Animals in the model group and the sham-operative group were clearly classified using the principal component analysis (PCA). The PCA of serum 1HNMR spectrum and metabolic spectrum in the two groups were significantly different in this model. Compared with the sham-operative group, endogenous metabolites in the serum such as the levels of citric acid, beta-hydroxybutyrate, gamma-aminobutyric acid, lactic acid, glutamate, glucose, etc. increased in the model group, while the concentrations of lipid metabolites such as low-density lipoprotein (LDL) and very low-density lipoprotein (VLDL) decreased.</p><p><b>CONCLUSIONS</b>The different metabolites between the model group and the sham-operative group mainly manifested as metabolic disorders of glucose and lipids, as well as energy metabolism such as the disturbance of tricarboxylic acid (TCA). Metabonomics could provide a new path for the objectivity of Chinese medicine syndromes.</p>