N-acetylcysteine promoted aging through adjusting expression of cell cycle related protein in neonatal SD rat cardiomyocytes / 中华心血管病杂志
Chinese Journal of Cardiology
; (12): 146-150, 2008.
Article
in Zh
| WPRIM
| ID: wpr-299481
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effects of N-acetylcysteine (NAC) on aging in neonatal SD rat cardiomyocytes and explore related mechanisms.</p><p><b>METHODS</b>Cultured cardiomyocytes were randomized assigned to 6 groups: 1-day, 5-day, 10-day, 1-day + NAC (1 mmol/L), 5-day + NAC (1 mmol/L) and 10-day + NAC (1 mmol/L). Flow cytometry was used to examine cell cycle. Real-time quantitative PCR and Western blot were used to determine mRNA and protein expression of p16INK4a, p21WAF1 and Rb gene. beta-galactosidase staining kit was used to investigate beta-galactosidase activity.</p><p><b>RESULTS</b>Numbers of cardiomyocytes resided in G(0)/G(1) phase were significantly higher in the group of 5-day + NAC and 10-day + NAC compared with 5-day, 10-day, respectively (P < 0.05). The mRNA and protein expression of p16INK4a and p21WAF1 were also significantly higher in the group of 5-day + NAC and 10-day + NAC compared with 5-day, 10-day, respectively (P < 0.05 or P < 0.01). The mRNA and protein expression of Rb was significantly lower in the group of 5-day + NAC and 10-day + NAC compared with 5-day, 10-day, respectively (P < 0.01). beta-galactosidase activity was not affected by NAC in the 1-day + NAC group but was significantly higher in 5-day + NAC and 10-day + NAC groups compared with the 5-day, 10-day groups (all P < 0.05).</p><p><b>CONCLUSION</b>NAC could promote aging through upregulating the expression of p16INK4a and p21WAF1 and inhibiting Rb phosphorylation in neonatal SD rat cardiomyocytes.</p>
Full text:
1
Database:
WPRIM
Main subject:
Pharmacology
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Acetylcysteine
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RNA, Messenger
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Cell Cycle
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Cells, Cultured
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Cellular Senescence
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Rats, Sprague-Dawley
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Cyclin-Dependent Kinase Inhibitor p16
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Myocytes, Cardiac
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Cyclin-Dependent Kinase Inhibitor p21
Limits:
Animals
Language:
Zh
Journal:
Chinese Journal of Cardiology
Year:
2008
Document type:
Article