Synergistic cytotoxicity of ampelopsin sodium and carboplatin in human non-small cell lung cancer cell line SPC-A1 by Gcell cycle arrested / 中国结合医学杂志
Chinese journal of integrative medicine
; (12): 125-131, 2017.
Article
in En
| WPRIM
| ID: wpr-301072
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the cytotoxic effects of ampelopsin sodium (Amp-Na) and carboplatin (CBP) used alone or in combination on human non-small cell lung cancer (NSCLC) cells SPC-A1 in vitro and its related mechanism.</p><p><b>METHODS</b>Cytotoxic effects were assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays. The synergistic effects of the drugs were calculated with coefficient of drug interaction (CDI). Cell cycle was determined by flow cytometry (FCM). The levels of p53, p21, cyclinE, cyclinD1, and phosphorylated cyclin-dependent kinase-2 (p-CDK2) were evaluated by Western blot.</p><p><b>RESULTS</b>Amp-Na (6.25-200 μg/mL) and CBP (3.13-100 μg/mL) alone exhibited prominent cytotoxic activity in a concentration-dependent manner on SPC-A1 cells with 50% inhibitive concentration values of 57.07±14.46 and 34.97±6.30 μg/mL, respectively. Drug combinations were associated with significantly higher cytotoxic effects than each drug alone (P<0.05 or 0.01). The CDI analysis confirmed the synergy of Amp-Na and CBP on inhibiting cancer cell viability across a wide concentration range (CDI <1). FCM and Western blot showed that synergistic cytotoxic effects of Amp-Na and CBP were related to Garrested which mainlym ediated by p 21 through the inhibition of CDK2 activity independent of the p53 tumor suppressor pathway.</p><p><b>CONCLUSIONS</b>Amp-Na exhibits anticancer activities and enhances the antitumor activities of CBP through up-regulation of p21 and inhibition of CDK2 activity in human NSCLC cells SPC-A1. These results suggest that Amp-Na may be applied to enhance the anticancer action of CBP.</p>
Key words
Full text:
1
Database:
WPRIM
Main subject:
Pathology
/
Pharmacology
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Flavonoids
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Antineoplastic Combined Chemotherapy Protocols
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Cell Cycle
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Carboplatin
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Carcinoma, Non-Small-Cell Lung
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Cell Line, Tumor
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Cell Proliferation
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Drug Synergism
Limits:
Humans
Language:
En
Journal:
Chinese journal of integrative medicine
Year:
2017
Document type:
Article