Relationship between M3 receptor and myocyte apoptosis induced by acute myocardial infarction / 药学学报
Acta Pharmaceutica Sinica
; (12): 338-341, 2004.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-301081
Responsible library:
WPRO
ABSTRACT
<p><b>AIM</b>To explore the effects of M3 receptor on myocyte apoptosis induced by acute myocardial infarction in rats.</p><p><b>METHODS</b>Rat model was induced by ligation of the anterior branch of the left coronary artery. All animals were divided into four groups sham-operated group, occlusion group, choline group (10 mg x kg(-1), iv), and 4DAMP (4-diphenylacetoxy-N-methylpiperidine-methiodide) group (0.12 mg x kg(-1), iv). The serum malondialdehyde (MDA) content and superoxide dismutase (SOD) activity were determined. The infarct size areas on the myocardium were identified by TTC staining. The apoptosis in cardiomyocyte was detected by TUNEL assay and apoptosis-related proteins in Bcl-2 and Fas expression were measured by immunohistochemistry assay.</p><p><b>RESULTS</b>M3 receptor agonist choline reduced serum MDA content and increased SOD activity. The myocardial expression of Bcl-2 was increased, whereas the expression of Fas was decreased by choline. However, blockade of M3 receptor by 4DAMP completely inhibited these effects of choline on cardiac myocytes.</p><p><b>CONCLUSION</b>Activation of M3 receptor has protective effect on myocyte apoptosis induced by acute myocardial infarction in rat, and this effect might be related to modulating the expression of some immediateearly genes including Bcl-2 and Fas.</p>
Full text:
Available
Health context:
SDG3 - Target 3.4 Reduce premature mortality due to noncommunicable diseases
Health problem:
Cardiovascular Disease
/
Ischemic Heart Disease
Database:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Piperidines
/
Superoxide Dismutase
/
Blood
/
Choline
/
Rats, Wistar
/
Apoptosis
/
Receptors, Tumor Necrosis Factor
/
Fas Receptor
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
Acta Pharmaceutica Sinica
Year:
2004
Document type:
Article