Isolation and structure identification of lignans from pine needles of pinus massoniana Lamb / 药学学报

ABSTRACT

<p><b>AIM</b>To study the chemical constituents from the water-extracts of pine needles of Pinus massoniana Lamb.</p><p><b>METHODS</b>Chromatographic techniques were used to separate and purify compounds. Their physico-chemical properties and spectral data (UV, IR, MS, 1H-1H, 13C-1H NMR, DEPT, HMBC etc.) were used to elucidate the structures.</p><p><b>RESULTS</b>Three lignans were isolated from the n-BuOH fraction of water-extracts. Their structures were identified as (7S,8R)-3',4,9'-tridihydroxy-4-methoxy- 9-O-shikimoyl-7,8-dihydrobenzofuran-1'-propylneolignan (massonianoid A, I), (7S,8R)-4,9-dihydroxy-3,3'-dimethoxy-7,8- dihydrobenzofuran-1'-propylneolignan (II) and 4,4',8-trihydroxy-4,4'-dimethoxy-9-lignanolide (III).</p><p><b>CONCLUSION</b>Compound I is a new compound. While II and III were isolated from this plant for the first time.</p>