TNF-β genetic variant contributes to the risk of non-small cell lung cancer / 中华预防医学杂志
Chinese Journal of Preventive Medicine
; (12): 41-44, 2015.
Article
in Zh
| WPRIM
| ID: wpr-302521
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the association of TNF-β 252A >G variant with the risk of non-small cell lung cancer (NSCLC).</p><p><b>METHODS</b>Total 956 patients with NSCLC were recruited between January 2000 and December 2008 at Cancer Hospital, Chinese Academy of Medical Science as the case group, and 994 frequency-matched controls were randomly selected from a pool of cancer-free subjects recruited from a nutritional group. All the participants were unrelated Han Chinese. There were no age, gender restrictions. Smoking status of the subjects was surveyed. Informed consent was obtained and 3 ml peripheral blood was collected from each subject. All samples were genotyped by polymerase chain reaction-restriction fragment length polymorphism method (PCR-RFLP). The OR and 95%CI were estimated by logistic regression to evaluate the relationship between TNF-β 252 A/G variant and the risk of lung cancer.</p><p><b>RESULTS</b>The frequencies of TNF-β 252 AA, AG and GG genotype were 30.9% (307/994) , 47.4% (471/994) and 21.7% (216/994) in lung cancer cases and 35.7% (341/956) , 48.1% (460/956) and 16.2% (155/956) in controls. Logistic regression analysis revealed that TNF-β 252 GG genotype contributed to a decreased risk of developing NSCLC (OR = 0.64, 95%CI: 0.49-0.83) compared with AA genotype. When stratified by smoking status, the individuals with 252 GG genotype had a significant increased risk of NSCLC (OR = 1.54, 95%CI:1.00-2.39) among smokers; which was less than those with AA genotype among smokers (OR = 2.88, 95%CI:1.91-2.24). When further stratified by smoking index, individuals with 252 GG genotype had a significant decreased risk of NSCLC among heavy smokers with OR (95%CI) of 2.24 (1.33-3.74), which was less than those with AA genotype (OR = 4.62, 95%CI:2.88-7.41).</p><p><b>CONCLUSION</b>TNF-β genetic variant may interact with environment factor to contribute to the susceptibility to NSCLC.</p>
Full text:
1
Database:
WPRIM
Main subject:
Polymorphism, Restriction Fragment Length
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Smoking
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Case-Control Studies
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China
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Polymerase Chain Reaction
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Risk
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Risk Factors
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Lymphotoxin-alpha
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Carcinoma, Non-Small-Cell Lung
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Genetic Predisposition to Disease
Type of study:
Etiology_studies
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Observational_studies
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Risk_factors_studies
Limits:
Humans
Country/Region as subject:
Asia
Language:
Zh
Journal:
Chinese Journal of Preventive Medicine
Year:
2015
Document type:
Article