C-jun N-terminal kinase-mediated signaling is essential for Staphylococcus aureus-induced U937 apoptosis / 中国医学科学杂志(英文版)
Chinese Medical Sciences Journal
; (4): 26-29, 2009.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-302655
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of SP600125, a specific c-jun N-terminal protein kinase (JNK) inhibitor, on Staphylococcus aureus (S. aureus)-induced U937 cell death and the underlying mechanism.</p><p><b>METHODS</b>The human monocytic U937 cells were treated with S. aureus at different time with or without SP600125. Cell apoptosis was analyzed by flow cytometry. JNK, Bax, and caspase-3 activities were detected by Western blotting.</p><p><b>RESULTS</b>S. aureus induced apoptosis in cultured U937 cells in a time-dependent manner. Expression of Bax and phospho-JNK significantly increased in S. aureus-treated U937 cells, and the level of activated caspase-3 also increased in a time-dependent manner. Inhibition of JNK with SP600125 significantly inhibited S. aureus-induced apoptosis in U937 cells.</p><p><b>CONCLUSIONS</b>S. aureus can induce apoptosis in U937 cells by phosphorylation of JNK and activation of Bax and caspase-3. SP600125 protects U937 cells from apoptosis induced by S. aureus via inhibiting the activity of JNK.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Phosphorylation
/
Physiology
/
Staphylococcus aureus
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Signal Transduction
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Apoptosis
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U937 Cells
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Cell Biology
/
JNK Mitogen-Activated Protein Kinases
/
Mitogen-Activated Protein Kinase 8
Limits:
Humans
Language:
English
Journal:
Chinese Medical Sciences Journal
Year:
2009
Document type:
Article