Determination of tramadol and its active metabolite O-desmethyltramadol in plasma and amniotic fluid using LC/MS/MS / 药学学报
Acta Pharmaceutica Sinica
; (12): 458-462, 2004.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-302786
Responsible library:
WPRO
ABSTRACT
<p><b>AIM</b>To determinate tramadol and O-desmethyltramadol in human plasma and amniotic fluid by LC/MS/MS, and distribution of tramadol and O-desmethyltramadol in maternity and fetus were studied.</p><p><b>METHODS</b>Samples containing tramadol, O-desmethyltramadol and diphenhydramine (internal standard, IS ) were extracted using liquid-liquid extraction, followed by liquid chromatographic separation and on-line MS/MS using atmospheric pressure chemical ionization as an interface detection. The analytes were detected in the selected reaction monitoring mode.</p><p><b>RESULTS</b>The calibration curves for tramadol and O-desmethytramadol in plasma and amniotic fluid were linear in the range from 8.0 to 800.0 microg x L(-1) (plasma) and 1.0 to 400.0 microg x L(-1) (amniotic fluid). The method was applied to the measurement of tramadol and O-desmethytramadol concentrations in maternal vein, umbilical vein, umbilical artery and amniotic fluid. Following intramuscular pre-operative administration 1.5 mg x kg(-1) doses of tramadol to parturients, plasma concentrations of tramadol were significantly higher than those in amniotic fluid. The concentrations of O-desmethyltramadol in plasma were lower, and were not detected in amniotic fluid.</p><p><b>CONCLUSION</b>The method is shown to be accurate, robust and convenient, and suitable for clinical pharmacokinetics studies of tramadol and O-desmethyltramadol.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Tramadol
/
Umbilical Arteries
/
Umbilical Veins
/
Blood
/
Pharmacokinetics
/
Chromatography, High Pressure Liquid
/
Spectrometry, Mass, Electrospray Ionization
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Fetus
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Amniotic Fluid
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Gas Chromatography-Mass Spectrometry
Limits:
Female
/
Humans
/
Pregnancy
Language:
Chinese
Journal:
Acta Pharmaceutica Sinica
Year:
2004
Document type:
Article