Effects of trxS gene on protein degradation in germinating barley seeds / 生物工程学报
Chinese Journal of Biotechnology
; (12): 84-88, 2009.
Article
in En
| WPRIM
| ID: wpr-302851
Responsible library:
WPRO
ABSTRACT
We assessed the effects of trxS gene on changes of proteinase activity, contents of different protein fractions and SDS-PAGE profiles in germinating seeds of contrasting transgenic and nontransgenic barley variety. Proteinase activity was enhanced by 70.28% in transgenic than nontransgenic barley seeds, whereas contents of albumin, globulin, hordein and glutelin in transgenic seeds were 3.68%, 23.52%, 31.37%, and 21.04%, lower than those in nontransgenic seeds. Degradation rates of hordein and glutelin in transgenic seeds were faster than those in nontransgenic seedlings as indicated by the SDS-PAGE profiles. Our data imply that the transformation of trxS gene could promote the degradation of protein, providing theoretic basis for the use of trxS gene and barley quality breeding.
Full text:
1
Database:
WPRIM
Main subject:
Peptide Hydrolases
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Plant Proteins
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Seeds
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Thioredoxins
/
Transformation, Genetic
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Hordeum
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Plants, Genetically Modified
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Germination
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Genetics
/
Metabolism
Language:
En
Journal:
Chinese Journal of Biotechnology
Year:
2009
Document type:
Article