Expression and identification of PEBP-like gene from Saussurea involucrate Kar.er kir in Escherichia coli / 生物工程学报
Chinese Journal of Biotechnology
; (12): 1649-1652, 2008.
Article
in Zh
| WPRIM
| ID: wpr-302906
Responsible library:
WPRO
ABSTRACT
This assay was designed to construct the prokaryotic expression vector, investigate the expression of PEBP-like in Escherichia coli and purify its product. The PEBP gene was inserted into the vector pET30a (+). The recombinant vector was transferred into E. coli BL21 (DE3)and induced the expression of protein by low concentration of IPTG and low temperature overnight. After purification, the supernatants were analyzed by SDS-PAGE and the results were identified by Western blotting. After IPTG induction, a new anticipating fusion protein of 28 kD appeared as an expected size, and its product was 26.8% in total protein, the fusion protein was positive by Western blotting. The prokaryotic expression system of PEBP-like is successfully constructed. It lays the foundation for the further application study on the antifreeze characters of the PEBP.
Full text:
1
Database:
WPRIM
Main subject:
Plant Proteins
/
Recombinant Proteins
/
Cloning, Molecular
/
Gene Expression Regulation, Plant
/
Saussurea
/
Phosphatidylethanolamine Binding Protein
/
Escherichia coli
/
Genetics
/
Metabolism
Type of study:
Diagnostic_studies
Language:
Zh
Journal:
Chinese Journal of Biotechnology
Year:
2008
Document type:
Article