Inhibitory effect of p53 gene on telomerase activity and proliferative activity in salivary adenoid cystic carcinoma cells / 中华口腔医学杂志
Chinese Journal of Stomatology
; (12): 45-48, 2006.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-303437
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the inhibitory effect of p53 gene on salivary adenoid cystic carcinoma (SACC) cells.</p><p><b>METHODS</b>Adenoviral vector pDeltaE1-p53 was constructed and transfected into SACC-83 cells. The enhanced p53 expression was measured by reverse transcription polymerase chain reaction (RT-PCR), and the effects of transfected p53 on SACC-83 cells were analyzed by TRAP-PCR-ELISA, luciferase reporter, flow cytometry (FCM), soft agar assay and tumorigenicity test.</p><p><b>RESULTS</b>The expression of p53 gene in SACC-83 cells was increased after introduction of pDeltaE1-p53. The telomerase activity and the transcriptional activity of hTERT promoter were inhibited. The cells cycles of transfected SACC-83 were arrested in G(1) phase and the rate of colony-formation was decreased, and similarly the tumorigenicity in nude mice was also decreased.</p><p><b>CONCLUSIONS</b>The introduction of wild-type p53 by adenoviral vector could suppress the telomerase activity and malignant phenotypes of salivary adenoid cystic carcinoma cells.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Salivary Gland Neoplasms
/
Transfection
/
Tumor Suppressor Protein p53
/
Carcinoma, Adenoid Cystic
/
Telomerase
/
Cell Line, Tumor
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Genetic Vectors
/
Genetics
/
Metabolism
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Stomatology
Year:
2006
Document type:
Article