Gene copy number, mRNA transcription and protein expression of PD-1 gene in primary hepatocarcinoma patients / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 282-284, 2012.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-305058
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the gene copy number, mRNA transcription and protien expression of programmed cell death 1 (PD-1) gene in primary hepatocellular carcinoma (PHC) patients and normal control individuals (NC) who are anti-HBs positive, and to investigate the variations in PD-1 gene copy numbers and its relationship with PHC.</p><p><b>METHODS</b>Real-time PCR was adopted to detect the PD-1 gene copy numbers and their mRNA expressions in peripheral blood mononuclear cells (PBMCs) from 24 samples of PHC patients and 26 of NC. Protein expression level of PD-1 on CD8+ T was analyzed by flow cytometry.</p><p><b>RESULTS</b>In terms of number of PD-1 gene copy numbers, the percentage of cases of haploid (single) was 34.62% and 4.17% in PHC group and control group respectively while the percentage of cases of diploid (double) was 61.54% and 95.83% respectively. The difference between the two was statistically significant (chi2 = 7.639, P = 0.006). The rate of cases with double PD-1 gene copy numbers was found to be higher in patients with PHC than in control group. It was also found that the average expression of PD-1 mRNA was 2.35E-03 in control group and 1.23E-03 in PHC group. The expression level was significant lower in PHC group than that in control group when compared by using Mann-whitey technic (U = 153, P = 0.009). Furthermore, the frequency of PD-1 protein expression on CD8+ T cells was 3.72 +/- 0.32 in control group and 16.13 +/- 1.68 in PHC group. The level of PD-1 mRNA expression was higher in PHC and significant differences was shown between two groups (t = -7.073, P = 0.000).</p><p><b>CONCLUSIONS</b>Our study suggests that the variation in PD-1 gene copy number may trigger primary hepatocellular carcinoma to HBV carriers. The relationship between the variation of PD-1 gene copy numbers and its association with primary hepatocellular carcinoma is worth further focus.</p>
Full text:
Available
Health context:
SDG3 - Target 3.4 Reduce premature mortality due to noncommunicable diseases
Health problem:
Digestive System Diseases
/
Liver Cancer
Database:
WPRIM (Western Pacific)
Main subject:
Transcription, Genetic
/
RNA, Messenger
/
Case-Control Studies
/
Gene Expression Regulation, Neoplastic
/
Carcinoma, Hepatocellular
/
Gene Dosage
/
Programmed Cell Death 1 Receptor
/
Genetics
/
Liver Neoplasms
/
Metabolism
Type of study:
Observational study
/
Risk factors
Limits:
Adult
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2012
Document type:
Article