Expression of T-bet and GATA3 in children with acute idiopathic thrombocytopenic purpura / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
; (12): 29-31, 2010.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-305115
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the expression of transcriptional factors T-bet and GATA3 mRNA and the levels of IFN-gamma and IL-4 in blood in children with acute idiopathic thrombocytopenic purpura (ITP) and investigate the tendency of polarization of Th1/Th2 in children with ITP.</p><p><b>METHODS</b>Blood T-bet and GATA3 mRNA expression were examined using RT-PCR and plasma IFN-gamma and IL-4 levels were measured using EIASA in children with acute ITP in acute (n=30) and remission stages (n=28). Twenty healthy children served as the controls.</p><p><b>RESULTS</b>Blood T-bet mRNA expression and IFN-gamma levels in children with ITP in the acute stage were markedly higher than those in the remission stage and controls (p<0.01). In contrast, blood GATA3 mRNA expression and IL-4 levels in children with ITP in the acute stage were significantly lower than those in the remmission stage and controls (p<0.01).</p><p><b>CONCLUSIONS</b>The high expression of T-bet and IFN-gamma and the low expression of GATA3 and IL-4 indicate the existence of Th1 polarization in children with acute ITP. This might be related to the regulation of T-bet and GATA3.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Blood
/
RNA, Messenger
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Interleukin-4
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Interferon-gamma
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Purpura, Thrombocytopenic, Idiopathic
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Th1 Cells
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T-Box Domain Proteins
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Allergy and Immunology
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GATA3 Transcription Factor
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Genetics
Limits:
Child
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Child, preschool
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Female
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Humans
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Male
Language:
Chinese
Journal:
Chinese Journal of Contemporary Pediatrics
Year:
2010
Document type:
Article