Regulative role of TNFalpha on STEAP4 gene in matured human adipocytes / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
; (12): 1008-1011, 2009.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-305133
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>Human STEAP4, a novel obesity-related gene, is associated with insulin sensitivity regulation in human adipocytes. This study aimed to explore the regulative role of TNFalpha on STEAP4 gene in matured human adipocytes.</p><p><b>METHODS</b>Human preadipocytes were cultured and differentiated into matured adipocytes in vitro. Fully differentiated adipocytes (Day 17) were treated with different concentrations of TNFalpha (0, 5, 10, 25 and 50 ng/mL) for 24 hrs. Total RNA and protein were extracted from the adipocytes. Levels of STEAP4 mRNA and protein expression were determined by real-time quantitative RT-PCR and Western blot respectively.</p><p><b>RESULTS</b>Different concentrations (5, 10, 25 and 50 ng/mL) of TNFalpha treatment for 24 hrs resulted in a significant increase in the STEAP4 mRNA expression of human matured adipocytes.The maximal effect was seen in the 50 ng/mL of TNFalpha treatment group. In parallel, STEAP4 protein synthesis in matured adipocytes increased in response to TNFalpha treatment of different concentrations (5, 10, 25 and 50 ng/mL) for 24 hrs. The maximal up-regulated effect was seen in the 25 ng/mL of TNFalpha treatment group.</p><p><b>CONCLUSIONS</b>TNFalpha can up-regulate STEAP4 mRNA expression in human matured adipocytes.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Oxidoreductases
/
Pharmacology
/
Recombinant Proteins
/
Cells, Cultured
/
Gene Expression Regulation
/
Tumor Necrosis Factor-alpha
/
Adipocytes
/
Dose-Response Relationship, Drug
/
Genetics
/
Membrane Proteins
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Contemporary Pediatrics
Year:
2009
Document type:
Article