Effects of SDS, PBS solutions upon fluorescence values of Dermatophagoides pterronyssinus radioallergosorbent test inhibition / 中国医学科学杂志(英文版)
Chinese Medical Sciences Journal
; (4): 27-29, 2005.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-305467
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effects of SDS, PBS re-dissolvent solutions on fluorescence values of radioallergosorbent test (RAST) inhibition.</p><p><b>METHODS</b>Dermatophagoides pterronyssinus allergen immunoCAP and UniCAP 100 System were used. The Sera Pool consisted of 20 Dermatophagoides pterronyssinus allergic patients sera, their specific IgE fluorescence values were between 12505 and 24776.</p><p><b>RESULTS</b>Fluorescence value percentages decreased 62.9%, 54.1%, 43.5%, 6.7%, 3.7%, 2.6%, 2.2%, and 1.4% respectively, when SDS concentrations were at 2%, 1%, 0.5%, 0.25%, 0.1%, 0.05%, 0.025%, and 0.01%. Fluorescence values decreased more than 5% with SDS concentrations equal to 0.25% or higher. PBS in 0.1 and 0.01 mol/L concentrations decreased fluorescence values 2.9% and 0.9% respectively.</p><p><b>CONCLUSIONS</b>SDS is a commonly used surfactants in allergen extract and re-dissolvent prepared allergen precipitation for RAST inhibition. Thus effects of surfactants (e.g. SDS) upon the RAST inhibition tests must be considered when they were used as re-dissolvent agents to improve protein resolution in RAST inhibition.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Phosphates
/
Sodium Dodecyl Sulfate
/
Solutions
/
Surface-Active Agents
/
Blood
/
Immunoglobulin E
/
Radioallergosorbent Test
/
Allergens
/
Dermatophagoides pteronyssinus
Limits:
Humans
Language:
English
Journal:
Chinese Medical Sciences Journal
Year:
2005
Document type:
Article