Specific immune responses in Balb/c mice induced by plasmid coexpressing hepatitis B surface antigen and granulocyte-macrophage colony stimulating factor / 中华肝脏病杂志
Chinese Journal of Hepatology
; (12): 474-476, 2003.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-305887
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVES</b>To investigate the improvement of specific immune responses induced by plasmid coexpressing hepatitis B surface antigen (HBsAg) and granulocyte-macrophage colony stimulating factor (GM-CSF).</p><p><b>METHODS</b>All Balb/c (H-2d) mice were immunized with pGM-CSF/S, pS/GM-CSF, pS or control plasmids. 4 weeks later, anti-HBs titer and the levels of IL-2, IL-4 and IFN-gamma in the supernatant of splenocytes were detected using enzyme- linked immunosorbent assay (ELISA), and HBsAg-specific cytotoxic T lymphocytes (CTL) activity was measured with a 51Cr release assay, using P815/S transfectants as target cells.</p><p><b>RESULTS</b>The anti-HBs antibody titers in the serum, the levels of IL-2 and IFN-gamma, and the CTL activity in pcDNA3.1-GM-CSF-S immuned mice were higher than those in PcDNA3.1-S immunized mice (F=4.176, P<0.01; F=31.188, P<0.01; F=31.796, P<0.01; F<or=26.891, P<0.01).</p><p><b>CONCLUSION</b>It will improve the specific immune responses induced by HBsAg DNA vaccine after it is binded to the gene of GM-CSF.</p>
Full text:
Available
Health context:
SDG3 - Health and Well-Being
Health problem:
Target 3.3: End transmission of communicable diseases
Database:
WPRIM (Western Pacific)
Main subject:
Enzyme-Linked Immunosorbent Assay
/
Lymphocyte Activation
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Granulocyte-Macrophage Colony-Stimulating Factor
/
Interleukin-4
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Interferon-gamma
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Interleukin-2
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Hepatitis B Vaccines
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Vaccines, DNA
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Allergy and Immunology
/
Genetics
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Hepatology
Year:
2003
Document type:
Article