Expression of epithelial-mesenchymal transition markers in tumor tissues generated by side population and non-side population of A431 cells in NOD/SCID mice / 南方医科大学学报
Journal of Southern Medical University
; (12): 733-737, 2013.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-306478
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To isolate the side population (SP) and non-side population (NSP) cells from A431 cells and compare their difference in tumorigenicity in mice and the expression profiles of epithelial-mesenchymal transition (EMT) markers.</p><p><b>METHODS</b>A431 cells stained with Hoechst 33342 were sorted with flow cytometry. The isolated SP cells and NSP cells were inoculated into NOD/SCID mice and the tumorigenicity of the cells was observed. EMT markers E-cadherin, β-catenin, vimentin, AXL, and Erbb3 in the tumor tissues were detected by immunohisto-staining.</p><p><b>RESULTS</b>The tumors generated by SP cells were larger than those by NSP cells in NOD/SCID mice. Compared with the tumors generated by NSP cells, the cells in the periphery of tumors generated by SP cells showed up-regulated expressions of AXL, vimentin and β-catenin and down-regulated ERBB3 and E-cadherin.</p><p><b>CONCLUSION</b>The SP cells in A431 cells have a strong tumorigenicity and show more EMT phenotypes in tissues.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Pathology
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Skin Neoplasms
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Neoplastic Stem Cells
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Carcinoma, Squamous Cell
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Biomarkers, Tumor
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Mice, SCID
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Mice, Inbred NOD
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Cell Line, Tumor
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Side-Population Cells
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Epithelial-Mesenchymal Transition
Limits:
Animals
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Humans
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Male
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2013
Document type:
Article