Protective mechanisms of sevoflurane against one-lung ventilation-induced acute lung injury: role of cyclooxygenase-2 and 5-lipoxygenase pathways / 南方医科大学学报
Journal of Southern Medical University
; (12): 625-630, 2013.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-306497
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the protective mechanisms of sevoflurane against acute lung injury (ALI) induced by one-lung ventilation (OLV) in view of cyclooxygenase-2 (COX2) and 5-lipoxygenase (5-LOX) pathways.</p><p><b>METHOD</b>Eighteen healthy Japanese white rabbits were randomized into sham-operated group (S group), OLV group (O group) and OLV + sevoflurane group (OS group). COX2 and 5-LOX protein and mRNA expressions in the lungs were detected by Western blotting and real-time PCR, respectively. Prostaglandin I2 (PGI2), thromboxane A2 (TXA2) and leukotrienes B2 (LTB2) in the lung tissues were quantified with ELISA. Histological scores and lung wet/dry weight (W/D) ratios were determined for lung injury assessment.</p><p><b>RESULTS</b>COX2 and 5-LOX protein and mRNA expressions and the contents of LTB2, TXA2 and PGI2 in the lungs, lung W/D ratio and histological scores were significantly higher while PGI2/TXA2 ratio was significantly lower in O group and OS group than in S group (P<0.05). Compared with those in O group, COX2 and 5-LOX expressions, pulmonary contents of LTB2, TXA2 and PGI2, and lung W/D ratio all decreased significantly but PGI2/TXA2 ratio was significantly elevated in OS group (P<0.05).</p><p><b>CONCLUSION</b>OLV may activate COX2 and 5-LOX pathways to result in increased production of arachidonic acid metabolites. Sevoflurane protects against OLV-induced ALI probably by reducing AA metabolites and regulating PGI2/TXA2 ratio through inhibitions of COX2 and 5-LOX pathways.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Arachidonate 5-Lipoxygenase
/
RNA, Messenger
/
Cyclooxygenase 2
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Acute Lung Injury
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One-Lung Ventilation
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Genetics
/
Lung
/
Metabolism
/
Methyl Ethers
Limits:
Animals
Language:
English
Journal:
Journal of Southern Medical University
Year:
2013
Document type:
Article